Research Papers: Imaging

Deep imaging in scattering media with selective plane illumination microscopy

[+] Author Affiliations
Adithya Kumar Pediredla, Shizheng Zhang, Ben Avants, Fan Ye, Ziying Chen

Rice University, Department of Electrical and Computer Engineering, 6100 Main Street, Houston, Texas 77005, United States

Shin Nagayama

The University of Texas Health Science Center at Houston, McGovern Medical School, 6431 Fannin Street, Houston, Texas 77030, United States

Caleb Kemere

Rice University, Department of Electrical and Computer Engineering, 6100 Main Street, Houston, Texas 77005, United States

Rice University, Department of Bioengineering, 6100 Main Street, Houston, Texas 77005, United States

Jacob T. Robinson

Rice University, Department of Electrical and Computer Engineering, 6100 Main Street, Houston, Texas 77005, United States

Rice University, Department of Bioengineering, 6100 Main Street, Houston, Texas 77005, United States

Baylor College of Medicine, Department of Neuroscience, 1 Baylor Plaza, Houston, Texas 77030, United States

Ashok Veeraraghavan

Rice University, Department of Electrical and Computer Engineering, 6100 Main Street, Houston, Texas 77005, United States

Rice University, Department of Computer Science, 6100 Main Street, Houston, Texas 77005, United States

J. Biomed. Opt. 21(12), 126009 (Dec 20, 2016). doi:10.1117/1.JBO.21.12.126009
History: Received August 26, 2016; Accepted November 21, 2016
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Abstract.  In most biological tissues, light scattering due to small differences in refractive index limits the depth of optical imaging systems. Two-photon microscopy (2PM), which significantly reduces the scattering of the excitation light, has emerged as the most common method to image deep within scattering biological tissue. This technique, however, requires high-power pulsed lasers that are both expensive and difficult to integrate into compact portable systems. Using a combination of theoretical and experimental techniques, we show that if the excitation path length can be minimized, selective plane illumination microscopy (SPIM) can image nearly as deep as 2PM without the need for a high-powered pulsed laser. Compared to other single-photon imaging techniques like epifluorescence and confocal microscopy, SPIM can image more than twice as deep in scattering media (10 times the mean scattering length). These results suggest that SPIM has the potential to provide deep imaging in scattering media in situations in which 2PM systems would be too large or costly.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Adithya Kumar Pediredla ; Shizheng Zhang ; Ben Avants ; Fan Ye ; Shin Nagayama, et al.
"Deep imaging in scattering media with selective plane illumination microscopy", J. Biomed. Opt. 21(12), 126009 (Dec 20, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.12.126009


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