Necrosis and apoptosis pathways of cell death at photodynamic treatment in vitro as revealed by digital holographic microscopy

I. V. Semenova; A. V. Belashov; T. N. Belyaeva; E. S. Kornilova; A. V. Salova; A. A. Zhikhoreva; O. S. Vasyutinskii

doi:10.1117/12.2287574

20 February 2018 Necrosis and apoptosis pathways of cell death at photodynamic treatment in vitro as revealed by digital holographic microscopy

I. V. Semenova, A. V. Belashov, T. N. Belyaeva, E. S. Kornilova, A. V. Salova, A. A. Zhikhoreva, O. S. Vasyutinskii

Author Affiliations +

Proceedings Volume 10497, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI; 104970D (2018) https://doi.org/10.1117/12.2287574
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Monitoring of variations in morphological characteristics of cultured HeLa cells after photodynamic treatment with Radachlorin photosensitizer is performed by means of digital holographic microscopy. The observed dose-dependent post-treatment variations of phase shift evidence threshold effect of photodynamic treatment and allow for distinguishing between necrotic or apoptotic pathways of cell death. Results obtained by holographic microscopy were confirmed by means of far-field optical microscopy and confocal fluorescence microscopy with commonly used test assays.

Citation Download Citation

I. V. Semenova, A. V. Belashov, T. N. Belyaeva, E. S. Kornilova, A. V. Salova, A. A. Zhikhoreva, and O. S. Vasyutinskii "Necrosis and apoptosis pathways of cell death at photodynamic treatment in vitro as revealed by digital holographic microscopy ", Proc. SPIE 10497, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI, 104970D (20 February 2018); https://doi.org/10.1117/12.2287574

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available