Multi-modal digital holographic microscopy for wide-field fluorescence and 3D phase imaging

Xiangyu Quan; Peng Xia; Osamu Matoba; Koichi Nitta; Yasuhiro Awatsuji

doi:10.1117/12.2212086

9 March 2016 Multi-modal digital holographic microscopy for wide-field fluorescence and 3D phase imaging

Xiangyu Quan, Peng Xia, Osamu Matoba, Koichi Nitta, Yasuhiro Awatsuji

Proceedings Volume 9718, Quantitative Phase Imaging II; 971821 (2016) https://doi.org/10.1117/12.2212086
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Multi-modal digital holographic microscopy is a combination of epifluorescence microscopy and digital holographic microscopy, the main function of which is to obtain images from fluorescence intensity and quantified phase contrasts, simultaneously. The proposed system is mostly beneficial to biological studies, with the reason that often the studies are depending on fluorescent labeling techniques to detect certain intracellular molecules, while phase information reflecting properties of unstained transparent elements. This paper is presenting our latest researches on applications such as randomly moving micro-fluorescent beads and living cells of Physcomitrella patens. The experiments are succeeded on obtaining a succession of wide-field fluorescent images and holograms from micro-beads, and different depths focusing is realized via numerical reconstruction. Living cells of Physcomitrella patens are recorded in the static manner, the reconstruction distance indicates thickness of cellular structure. These results are implementing practical applications toward many biomedical science researches.

Conference Presentation

Citation Download Citation

Xiangyu Quan, Peng Xia, Osamu Matoba, Koichi Nitta, and Yasuhiro Awatsuji "Multi-modal digital holographic microscopy for wide-field fluorescence and 3D phase imaging", Proc. SPIE 9718, Quantitative Phase Imaging II, 971821 (9 March 2016); https://doi.org/10.1117/12.2212086

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