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24 April 2018 Optical trapping of quantum-dot conjugated AMPA-type receptors depend on initial assembling states
Tatsunori Kishimoto, Yasuyo Maezawa, Suguru N. Kudoh, Takahisa Taguchi, Chie Hosokawa
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Proceedings Volume 10712, Optical Manipulation Conference; 1071204 (2018) https://doi.org/10.1117/12.2319362
Event: SPIE Structured Light, 2018, Yokohama, Japan
Abstract
AMPA-type glutamate receptor (AMPAR) is one of the major neurotransmitter receptors at excitatory synapses. The initial assembling states of AMPARs at cell surface are essential for synaptic transmission, which is related with learning and memory in living neural systems. To realize artificial control of synaptic transmission, we demonstrate to modulate the initial assembling states of quantum-dot conjugated AMPARs (QD-AMPARs) with optical trapping. The optical trapping dynamics of QD-AMPARs on living neurons was evaluated with fluorescence imaging and fluorescence correlation spectroscopy (FCS). The transit time at laser focus of QD-AMPARs on neurons estimated from FCS analysis increased with the culturing days and addition of neurotransmitter, which suggests that QD-AMPARs are confined at the focal spot due to optical trapping.
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Tatsunori Kishimoto, Yasuyo Maezawa, Suguru N. Kudoh, Takahisa Taguchi, and Chie Hosokawa "Optical trapping of quantum-dot conjugated AMPA-type receptors depend on initial assembling states", Proc. SPIE 10712, Optical Manipulation Conference, 1071204 (24 April 2018); https://doi.org/10.1117/12.2319362
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KEYWORDS
Neurons
Optical tweezers
Luminescence
Receptors
Fluorescence correlation spectroscopy
Neurotransmitters
Synaptic transmission
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