In vivo multiphoton dynamic imaging of the hepatobiliary metabolism in chronic hepatic diseases

Hsu-Cheng Huang; Chih-Ju Lin; Sheng-Lin Lee; Wei-Hsiang Wang; Vladimir A. Hovhannisyan; Yao-De Huang; Hsuan-Shu Lee; Chen-Yuan Dong

doi:10.1117/12.2507345

22 February 2019 In vivo multiphoton dynamic imaging of the hepatobiliary metabolism in chronic hepatic diseases

Hsu-Cheng Huang, Chih-Ju Lin, Sheng-Lin Lee, Wei-Hsiang Wang, Vladimir A. Hovhannisyan, Yao-De Huang, Hsuan-Shu Lee, Chen-Yuan Dong

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Proceedings Volume 10882, Multiphoton Microscopy in the Biomedical Sciences XIX; 108821B (2019) https://doi.org/10.1117/12.2507345
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Intravital multiphoton microscopy was used to study hepatobiliary metabolism in chronic pathologies of the liver. Through the use of the probe molecule 6-carboxyfluorescein diacetate (6-CFDA), the effects of liver fibrosis, fatty liver, and hepatocellular carcinoma on the metabolic capabilities of mouse liver were investigated. After the acquisition of time lapse images, a first order kinetic model was used to calculate rate constant resolved images of various pathologies. It was found the ability of the liver to metabolically process the probe molecules varies among different pathologies, with liver fibrosis and fatty liver disease negatively impacted the uptake, processing, and excretion of molecules.

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Hsu-Cheng Huang, Chih-Ju Lin, Sheng-Lin Lee, Wei-Hsiang Wang, Vladimir A. Hovhannisyan, Yao-De Huang, Hsuan-Shu Lee, and Chen-Yuan Dong "In vivo multiphoton dynamic imaging of the hepatobiliary metabolism in chronic hepatic diseases", Proc. SPIE 10882, Multiphoton Microscopy in the Biomedical Sciences XIX, 108821B (22 February 2019); https://doi.org/10.1117/12.2507345

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KEYWORDS

Liver

Mode conditioning cables

In vivo imaging

Tumors

Pathology

Tumor growth modeling

Luminescence

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