Identifying classic and florid lobular carcinoma in situ using multiphoton microscopy

Tingfeng Shen; Jiajia He; Deyong Kang; Gangqin Xi; Zhong Chen; Wenjiao Ren; Jianxin Chen

doi:10.1117/12.2548596

20 December 2019 Identifying classic and florid lobular carcinoma in situ using multiphoton microscopy

Tingfeng Shen, Jiajia He, Deyong Kang, Gangqin Xi, Zhong Chen, Wenjiao Ren, Jianxin Chen

Proceedings Volume 11209, Eleventh International Conference on Information Optics and Photonics (CIOP 2019); 112092T (2019) https://doi.org/10.1117/12.2548596
Event: Eleventh International Conference on Information Optics and Photonics (CIOP 2019), 2019, Xi'an, China

Abstract

Florid type is a kind of morphological variation of lobular carcinoma in situ (LCIS). Florid lobular carcinoma in situ (FLCIS) has the same cytological features as LCIS, often associated with comedo-type necrosis. Unlike classic lobular carcinoma in situ (CLCIS), which is often managed with close observation or chemoprevention, the treatment guidelines recommend that FLCIS be managed in the same way as ductal carcinoma in situ (DCIS). Therefore, it is critical to accurately identify FLCIS for management purposes. Recently, multiphoton microscopy (MPM) has become a powerful imaging tool for label-free detection of biological tissue. In this work, we obtain high-resolution images of unstained normal and diseased breast specimens by MPM. In order to verify the imaging details, we also obtained hematoxylin-eosin (H and E) stained images of corresponding tissues to compare with the MPM images. Our results indicate that MPM can identify FLCIS and CLCIS through histological characteristics, including cell morphology and collagen structure. With the further improvement of MPM, its diagnostic capabilities of real-time and non-invasive may provide a new option for early detection of breast tumor

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Tingfeng Shen, Jiajia He, Deyong Kang, Gangqin Xi, Zhong Chen, Wenjiao Ren, and Jianxin Chen "Identifying classic and florid lobular carcinoma in situ using multiphoton microscopy", Proc. SPIE 11209, Eleventh International Conference on Information Optics and Photonics (CIOP 2019), 112092T (20 December 2019); https://doi.org/10.1117/12.2548596

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KEYWORDS

Breast

Collagen

Second-harmonic generation

Multiphoton microscopy

Breast cancer

Tumors

Laser applications

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