Fluorescent proteins require reactive oxygen species to develop fluorescence

Pavel A. Savitsky; Yulii A. Labas; Alexander P. Savitsky

doi:10.1117/12.479829

12 September 2003 Fluorescent proteins require reactive oxygen species to develop fluorescence

Pavel A. Savitsky, Yulii A. Labas, Alexander P. Savitsky

Proceedings Volume 4967, Genetically Engineered and Optical Probes for Biomedical Applications; (2003) https://doi.org/10.1117/12.479829
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

The green fluorescent protein (GFP) from jellyfish Aequorea victoria is a useful tool in biochemistry and cell biology due to its ability to develop the fluorescence in wide variety of organisms. This indicates that maturation of GFP does not require any specific enzymes from jellyfish and chromophore formation may be spontaneous or dependent on ubiquitous factors. There is a great progress in elucidation of mechanism of this process since the GFP was discovered. Nevertheless, the last step of chromophore formation, the oxygen dependent dehydration of Ä„-Äñ bond of Tyr66, still remains unclear. We proposed that this dehydration require a reactive oxygen species (ROS) and arise through the hydroxylation of CÄñ atom of Tyr66 followed by the water elimination and formation of double bond. Recently six novel fluorescent proteins (FPs) have been cloned. We have found that maturation of these FPs in E.coli can be dramatically accelerate by addition of paraquat (superoxide generator).

Citation Download Citation

Pavel A. Savitsky, Yulii A. Labas, and Alexander P. Savitsky "Fluorescent proteins require reactive oxygen species to develop fluorescence", Proc. SPIE 4967, Genetically Engineered and Optical Probes for Biomedical Applications, (12 September 2003); https://doi.org/10.1117/12.479829

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