In vitro human atherosclerotic plaque recognition by photosensitizer mono-L-aspartyl chlorin e6-assisted light-induced fluorescence (PALIF)

Dariusz Bialy; Arkadiusz Derkacz; M. Wawrzynska; A. Bednarkiewicz; Piotr P. Ziolkowski; H. Nowosad; Wieslaw Strek; Marcin Protasiewicz

doi:10.1117/12.520616

6 October 2003 In vitro human atherosclerotic plaque recognition by photosensitizer mono-L-aspartyl chlorin e6 assisted light induced fluorescence (PALIF)

Dariusz Bialy, Arkadiusz Derkacz, M. Wawrzynska, A. Bednarkiewicz, Piotr P. Ziolkowski, H. Nowosad, Wieslaw Strek, Marcin Protasiewicz

Author Affiliations +

Proceedings Volume 5229, Laser Technology VII: Applications of Lasers; (2003) https://doi.org/10.1117/12.520616
Event: Laser Technology VII: Applications of Lasers, 2002, Szczecin, Poland

Abstract

The main aim of the presented experiments was to assess in vitro capabilities of Photosensitizer Assisted Light Induced Fluorescence (PALIF) to recognise different stages of atherosclerosis. Mono-L-asparyl-chlorin e-6 was used as a photosensitising agent and ultraviolet light (440 nm) as an excitation source to obtain spectra map of artery wall. The luminescence spectra were collected and analised. The specimans were histologically examined and classified into three groups: normal artery wall, atherosclerotic noncalcified plaque and calcified plaque. The ratio of green fluorescence (coming from collagen) to red fluorescence (coming from chlorin e6 bonded with lipid reach, noncalcified tissue) gies a chance to distinguish between normal artery or calcified plaque and noncalcified, lipid reach plaque. Further trials must be performed to evaluate in vitro athermoa detection algorithm as a feedback system for photoangioplasty.

Citation Download Citation

Dariusz Bialy, Arkadiusz Derkacz, M. Wawrzynska, A. Bednarkiewicz, Piotr P. Ziolkowski, H. Nowosad, Wieslaw Strek, and Marcin Protasiewicz "In vitro human atherosclerotic plaque recognition by photosensitizer mono-L-aspartyl chlorin e6 assisted light induced fluorescence (PALIF)", Proc. SPIE 5229, Laser Technology VII: Applications of Lasers, (6 October 2003); https://doi.org/10.1117/12.520616

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