CARS microscopy of Alzheimer's diseased brain tissue

Annika Enejder; Juris Kiskis; Helen Fink; Lena Nyberg; Jakob Thyr; Jia-Yi Li

doi:10.1117/12.2040915

28 February 2014 CARS microscopy of Alzheimer's diseased brain tissue

Annika Enejder, Juris Kiskis, Helen Fink, Lena Nyberg, Jakob Thyr, Jia-Yi Li

Proceedings Volume 8948, Multiphoton Microscopy in the Biomedical Sciences XIV; 89480U (2014) https://doi.org/10.1117/12.2040915
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Alzheimer’s disease (AD) is a progressive neurodegenerative disorder currently without cure, characterized by the presence of extracellular plaques surrounded by dystrophic neurites. In an effort to understand the underlying mechanisms, biochemical analysis (protein immunoblot) of plaque extracts reveals that they consist of amyloid-beta (Aβ) peptides assembled as oligomers, protofibrils and aggregates. Their spatial distribution has been confirmed by Thioflavin-S or immuno-staining with fluorescence microscopy. However, it is increasingly understood that the protein aggregation is only one of several mechanism that causes neuronal dysfunction and death. This raises the need for a more complete biochemical analysis. In this study, we have complemented 2-photon fluorescence microscopy of Thioflavin-S and Aβ immuno-stained human AD plaques with CARS microscopy. We show that the chemical build-up of AD plaques is more complex and that Aβ staining does not provide the complete picture of the spatial distribution or the molecular composition of AD plaques. CARS images provide important complementary information to that obtained by fluorescence microscopy, motivating a broader introduction of CARS microscopy in the AD research field.

Citation Download Citation

Annika Enejder, Juris Kiskis, Helen Fink, Lena Nyberg, Jakob Thyr, and Jia-Yi Li "CARS microscopy of Alzheimer's diseased brain tissue", Proc. SPIE 8948, Multiphoton Microscopy in the Biomedical Sciences XIV, 89480U (28 February 2014); https://doi.org/10.1117/12.2040915

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