A hybrid digital holographic microscopy for biological applications

O. Matoba; X. Quan; P. Xia; Y. Awatsuji

doi:10.1117/12.2185665

29 July 2015 A hybrid digital holographic microscopy for biological applications

O. Matoba, X. Quan, P. Xia, Y. Awatsuji

Proceedings Volume 9659, International Conference on Photonics Solutions 2015; 965903 (2015) https://doi.org/10.1117/12.2185665
Event: International Conference on Photonics Solutions 2015, 2015, Hua Hin, Thailand

Abstract

It will be useful in biological applications if two or more physical parameters are simultaneously measured in a digital holographic microscope. In this paper, we present a hybrid digital holographic microscope that can measure simultaneously three-dimensional (3D) phase and 3D fluorescence distributions. This property has big advantage compared with conventional optical microscopes such as phase contrast microscope and fluorescence microscope. We present an optical setup for measuring both phase and fluorescence images. In the experiments, two objects that are fluorescence beads and egera densa are used. The separation method of phase and fluorescence images is presented.

Citation Download Citation

O. Matoba, X. Quan, P. Xia, and Y. Awatsuji "A hybrid digital holographic microscopy for biological applications", Proc. SPIE 9659, International Conference on Photonics Solutions 2015, 965903 (29 July 2015); https://doi.org/10.1117/12.2185665

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