The effect of red light irradiation on spermatozoa DNA

Kay W. Chow; Daryl Preece; Veronica Gomez-Godinez; Michael W. Berns

doi:10.1117/12.2238182

16 September 2016 The effect of red light irradiation on spermatozoa DNA

Kay W. Chow, Daryl Preece, Veronica Gomez-Godinez, Michael W. Berns

Proceedings Volume 9922, Optical Trapping and Optical Micromanipulation XIII; 99222P (2016) https://doi.org/10.1117/12.2238182
Event: SPIE Nanoscience + Engineering, 2016, San Diego, California, United States

Abstract

A key goal in the conservation of endangered species is to increase successful reproduction. In cases where traditional methods of in vitro fertilization are unsuccessful, new methods of assisted reproduction are needed. One option is selective fertilization via optically trapped sperm. A more passive option is red light irradiation. Red light irradiation has been shown to increase sperm motility, thus increasing fertilizing potential. However, there is some concern that exposure to laser irradiation induces the production of oxidative species in cells, which can be damaging to DNA. In order to test the safety of irradiating sperm, sperm samples were exposed to 633 nm laser light and their DNA were tested for oxidative damage. Using fluorescence microscopy, antibody staining, and ELISA to detect oxidative DNA damage, it was concluded that red light irradiation does not pose a safety risk to sperm DNA. The use of red light on sperm has potential in both animal conservation and human reproduction techniques. This method can also be used in conjunction with optical trapping for viable sperm selection.

Citation Download Citation

Kay W. Chow, Daryl Preece, Veronica Gomez-Godinez, and Michael W. Berns "The effect of red light irradiation on spermatozoa DNA", Proc. SPIE 9922, Optical Trapping and Optical Micromanipulation XIII, 99222P (16 September 2016); https://doi.org/10.1117/12.2238182

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KEYWORDS

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DNA profiling

Detection and tracking algorithms

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Safety

Optical tweezers

Algorithm development

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