Dr. Khaled Youssef Profile

Dr. Khaled Youssef

at York Univ

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Publications (2)

Proceedings Article | 4 March 2022 Presentation + Paper

Optofluidic device for light-sheet fluorescence microscopy of C. elegans with a conventional wide-field microscope

Mehran Behrouzi, Faraz Rahimpouresfahani, Khaled Youssef, Pouya Rezai, Nima Tabatabaei

Proceedings Volume 11964, 1196402 (2022) https://doi.org/10.1117/12.2607134

KEYWORDS: Microscopes, Optofluidics, Imaging systems, Luminescence, Microscopy, Microfluidics, Confocal microscopy, Confocal fluorescent microscopy, Fluorescent proteins

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SPIE Journal Paper | 10 December 2021 Open Access

Low-cost optofluidic add-on enables rapid selective plane illumination microscopy of C. elegans with a conventional wide-field microscope

Mehran Behrouzi, Khaled Youssef, Pouya Rezai, Nima Tabatabaei

JBO, Vol. 26, Issue 12, 126501, (December 2021) https://doi.org/10.1117/12.10.1117/1.JBO.26.12.126501

KEYWORDS: Optofluidics, Microscopes, Imaging systems, Neurons, Microscopy, Visualization, Microfluidics, Objectives, Nerve, Image resolution

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Significance: Selective plane illumination microscopy (SPIM) is an emerging fluorescent imaging technique suitable for noninvasive volumetric imaging of C. elegans. These promising microscopy systems, however, are scarce in academic and research institutions due to their high cost and technical complexities. Simple and low-cost solutions that enable conversion of commonplace wide-field microscopes to rapid SPIM platforms promote widespread adoption of SPIM by biologist for studying neuronal expressions of C. elegans. Aim: We sought to develop a simple and low-cost optofluidic add-on device that enables rapid and immobilization-free volumetric SPIM imaging of C. elegans with conventional fluorescent microscopes. Approach: A polydimethylsiloxane (PDMS)-based device with integrated optical and fluidic elements was developed as a low-cost and miniaturized SPIM add-on for the conventional wide-field microscope. The developed optofluidic chip contained an integrated PDMS cylindrical lens for on-chip generation of the light-sheet across a microchannel. Cross-sectional SPIM images of C. elegans were continuously acquired by the native objective of microscope as worms flowed in an L-shape microchannel and through the light sheet. Results: On-chip SPIM imaging of C. elegans strains demonstrated possibility of visualizing the entire neuronal system in few seconds at single-neuron resolution, with high contrast and without worm immobilization. Volumetric visualization of neuronal system from the acquired cross-sectional two-dimensional images is also demonstrated, enabling the standard microscope to acquire three-dimensional fluorescent images of C. elegans. The full-width at half-maximum width of the point spread function was measured as 1.1 and 2.4 μm in the lateral and axial directions, respectively. Conclusion: The developed low-cost optofluidic device is capable of continuous SPIM imaging of C. elegans model organism with a conventional fluorescent microscope, at high speed, and with single neuron resolution.

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