Research Papers

Comparison of 1540-nm laser-induced injuries in ex vivo and in vitro rabbit corneal models

[+] Author Affiliations
Nicole A. McPherson

Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814

Thomas E. Eurell

Colorado State University, Fort Collins, Colorado 80523

Thomas E. Johnson

Colorado State University, Fort Collins, Colorado 80523

J. Biomed. Opt. 12(6), 064033 (December 18, 2007). doi:10.1117/1.2822371
History: Received March 27, 2007; Revised June 26, 2007; Published December 18, 2007
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Despite the increasing use of infrared lasers in medical, industrial, and military settings, data on threshold radiant exposures and median effective dose (ED50) as they relate to laser-tissue interaction are limited. Our goals were to determine the ED50 for single-pulse, 1540-nm laser exposures in ex vivo and in vitro rabbit corneal models and to characterize the histopathological changes associated with the laser-tissue interaction. An erbium-glass laser was used to deliver single, 1540-nm wavelength pulses to 27 ex vivo and 24 in vitro rabbit corneal models. The ex vivo model was exposed to single pulses of 0.8-ms duration and radiant energies ranging from 17.61Jcm2 to 42.26Jcm2. The in vitro corneal models were exposed to single pulses of 0.8ms duration and had radiant exposures ranging from 14.87 to 29.72Jcm2. Tissue exposure sites were observed for presence of a lesion immediately post-exposure and 24h after exposure. Histopathological evaluations of tissue exposure sites were conducted 24h after exposure. The ED50 was determined to be 21.24Jcm2 for the in vitro rabbit corneal models and 30.86Jcm2 for the ex vivo corneal models. Both the in vitro and ex vivo models displayed similar histopathological responses of tissue necrosis and epithelial cell proliferation.

© 2007 Society of Photo-Optical Instrumentation Engineers

Topics

Lasers ; Tissues ; Injuries

Citation

Nicole A. McPherson ; Thomas E. Eurell and Thomas E. Johnson
"Comparison of 1540-nm laser-induced injuries in ex vivo and in vitro rabbit corneal models", J. Biomed. Opt. 12(6), 064033 (December 18, 2007). ; http://dx.doi.org/10.1117/1.2822371


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