Label-free visible wavelength transient absorption imaging: mitochondrial redox contrast and unsupervised spectral learning

Jesse W. Wilson; Erkang Wang; Arya Mugdha; Luke Whitcomb; Adam Chicco

doi:10.1117/12.2634778

3 October 2022 Label-free visible wavelength transient absorption imaging: mitochondrial redox contrast and unsupervised spectral learning

Jesse W. Wilson, Erkang Wang, Arya Mugdha, Luke Whitcomb, Adam Chicco

Author Affiliations +

Proceedings Volume PC12228, Ultrafast Nonlinear Imaging and Spectroscopy X; PC122280P (2022) https://doi.org/10.1117/12.2634778
Event: SPIE Optical Engineering + Applications, 2022, San Diego, California, United States

Abstract

Transient absorption microscopy (TAM) provides imaging contrast from absorptive pigments such as hemeproteins and melanin, based on femtosecond to picosecond-timescale relaxation dynamics. TAM operates by exciting the sample with a short pump pulse, then measuring the time-dependent change in optical absorption, after excitation, with a probe pulse. Here we show that a 520nm pump and 620nm probe provides label-free imaging contrast for hemoglobin, myoglobin, and respiratory chain hemes of mitochondria with sensitivity to redox. We also introduce a simple convolutional neural network for analysis of TAM stacks. Finally, we will discuss future clinical applications to mitochondrial disease.

Conference Presentation

Citation Download Citation

Jesse W. Wilson, Erkang Wang, Arya Mugdha, Luke Whitcomb, and Adam Chicco "Label-free visible wavelength transient absorption imaging: mitochondrial redox contrast and unsupervised spectral learning", Proc. SPIE PC12228, Ultrafast Nonlinear Imaging and Spectroscopy X, PC122280P (3 October 2022); https://doi.org/10.1117/12.2634778

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