Elucidating biophysical mechanisms of directed energy exposure using quantitative phase imaging

Zachary A. Steelman; Sean O'Connor; Anna Sedelnikova; Matthew Conway; Stacey Martens; Joel Bixler

doi:10.1117/12.3012964

10 June 2024 Elucidating biophysical mechanisms of directed energy exposure using quantitative phase imaging

Zachary A. Steelman, Sean O'Connor, Anna Sedelnikova, Matthew Conway, Stacey Martens, Joel Bixler

Proceedings Volume PC13041, Three-Dimensional Imaging, Visualization, and Display 2024; PC1304107 (2024) https://doi.org/10.1117/12.3012964
Event: SPIE Defense + Commercial Sensing, 2024, National Harbor, Maryland, United States

Abstract

Single-cell analysis, or cytometry, is a ubiquitous tool in the biomedical sciences. Whereas most cytometers use fluorescent probes to ascertain the presence or absence of targeted molecules, biophysical parameters such as the cell density, refractive index, and water content are difficult to obtain. We present quantitative phase imaging as an effective technique to quantify the absolute intracellular water content in single cells at video rate, using an assumption of a spherical cellular geometry. Our study demonstrates the utility of QPI for rapid intracellular water quantification and shows a path forward for identifying biophysical mechanisms using label-free imaging. We further demonstrate the use of two complementary techniques - quantitative phase imaging and Brillouin spectroscopy—as a label-free image cytometry platform capable of measuring more than a dozen biophysical properties of individual cells simultaneously. Our system will unlock new avenues of research in biophysics, cell biology, and medicine.

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Citation Download Citation

Zachary A. Steelman, Sean O'Connor, Anna Sedelnikova, Matthew Conway, Stacey Martens, and Joel Bixler "Elucidating biophysical mechanisms of directed energy exposure using quantitative phase imaging", Proc. SPIE PC13041, Three-Dimensional Imaging, Visualization, and Display 2024, PC1304107 (10 June 2024); https://doi.org/10.1117/12.3012964

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KEYWORDS

Phase imaging

Directed energy weapons

Biological imaging

Water

Biomedical optics

Refractive index

Viscosity

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