QUANTITATIVE ENZYMATIC AND IMMUNOLOGIC HISTOPHOTOMETRY OF DISEASED HUMAN KID-NEY TISSUES USING TV-CAMERA AND COMPUTER ASSISTED IMAGE PROCESSING SYSTEMS.

G. Heinert; W. Mondorf

doi:10.1117/12.934635

1 November 1982 QUANTITATIVE ENZYMATIC AND IMMUNOLOGIC HISTOPHOTOMETRY OF DISEASED HUMAN KID-NEY TISSUES USING TV-CAMERA AND COMPUTER ASSISTED IMAGE PROCESSING SYSTEMS.

G. Heinert, W. Mondorf

Author Affiliations +

Proceedings Volume 0375, Medical Imaging and Image Interpretation; (1982) https://doi.org/10.1117/12.934635
Event: 1st International Symposium on Medical Imaging and Image Interpretation, 1982, Berlin, Germany

Abstract

High speed image processing was used to analyse morphologic and metabolic characteristics of clinically relevant kidney tissue alterations.Qualitative computer-assisted histophotometry was performed to measure alterations in levels of the enzymes alkaline phosphatase (Ap),alanine aminopeptidase (AAP),g-glutamyltranspepti-dase (GGTP) and A-glucuronidase (B-G1) and AAP and GGTP immunologically determined in prepared renal and cancer tissue sections. A "Mioro-Videomat 2" image analysis system with a "Tessovar" macroscope,a computer-assisted "Axiomat" photomicroscope and an "Interactive Image Analysis System (IBAS)" were employed for analysing changes in enzyme activities determined by changes in absorbance or transmission.Diseased kidney as well as renal neoplastic tissues could be distinguished by significantly (wilcoxon test,p<0,05) decreased enzyme concentrations as compared to those found in normal human kidney tissues.This image analysis techniques might be of potential use in diagnostic and prognostic evaluation of renal cancer and diseased kidney tissues.

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G. Heinert and W. Mondorf "QUANTITATIVE ENZYMATIC AND IMMUNOLOGIC HISTOPHOTOMETRY OF DISEASED HUMAN KID-NEY TISSUES USING TV-CAMERA AND COMPUTER ASSISTED IMAGE PROCESSING SYSTEMS.", Proc. SPIE 0375, Medical Imaging and Image Interpretation, (1 November 1982); https://doi.org/10.1117/12.934635

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