Fiber Optic Immunodetectors: Sensors Or Dosimeters?

J D Andrade; J-N Lin; J Herron; M Reichert; J Kopecek

doi:10.1117/12.937528

28 April 1987 Fiber Optic Immunodetectors: Sensors Or Dosimeters?

J D Andrade, J-N Lin, J Herron, M Reichert, J Kopecek

Proceedings Volume 0718, Fiber Optic and Laser Sensors IV; (1987) https://doi.org/10.1117/12.937528
Event: Cambridge Symposium-Fiber/LASE '86, 1986, Cambridge, MA, United States

Abstract

Biosensors based on specific interaction of the analyte of interest (ligand) with a biochemical agent (receptor) which specifically and tightly binds the analyte are being developed by many groups, agencies, and institutions using a variety of detection technologies. We are studying fluorescent methods using evanescent wave excitation on the surface of planar and cylindrical (fiber optic) optical waveguides. A relatively low ligand-receptor binding constant results in reversibility but low sensitivity. Most biological receptors bind very strongly and specifically--almost irreversibly--which provides an "irreversible" means of detection. Means to regulate ligand-receptor binding constants are discussed in order to provide semi-reversible, sensitive, specific sensors, rather than "one-shot" detectors or dosimeters.

Citation Download Citation

J D Andrade, J-N Lin, J Herron, M Reichert, and J Kopecek "Fiber Optic Immunodetectors: Sensors Or Dosimeters?", Proc. SPIE 0718, Fiber Optic and Laser Sensors IV, (28 April 1987); https://doi.org/10.1117/12.937528

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