Research on detecting plankton based on darkfield microscopy

Buyu Guo; Jia Yu; Huiping Liu; Xiaoying Liu; Rui Hou

doi:10.1117/12.2246238

31 October 2016 Research on detecting plankton based on darkfield microscopy

Buyu Guo, Jia Yu, Huiping Liu, Xiaoying Liu, Rui Hou

Proceedings Volume 10021, Optical Design and Testing VII; 100211M (2016) https://doi.org/10.1117/12.2246238
Event: SPIE/COS Photonics Asia, 2016, Beijing, China

Abstract

Plankton is an important part of the marine life, which plays a significant role in studying the change of global climate and the marine ecological environment. In order to detect plankton accurately and effectively, we design the Underwater In-situ Dark-field Microscopy Detection System based on the principle of dark-field microscopy. The experiment results show that while the 2× microscope objective is used, the resolution is 90.5lp/mm and the horizontal field of view is 2.6×2.6mm and while the 10× microscope objective is used, the resolution is 181lp/mm and the horizontal field of view is 2.2×2.2mm, when the microscope objective is replaced by 0.9× large format telecentric lens, the detection area will be 11mm with the resolution of 32lp/mm. Double compartment structure is designed, such that the system can work underwater.

Citation Download Citation

Buyu Guo, Jia Yu, Huiping Liu, Xiaoying Liu, and Rui Hou "Research on detecting plankton based on darkfield microscopy", Proc. SPIE 10021, Optical Design and Testing VII, 100211M (31 October 2016); https://doi.org/10.1117/12.2246238

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