Molecular dynamics in an optical trap of glutamate receptors labeled with quantum-dots on living neurons

Tatsunori Kishimoto; Yasuyo Maezawa; Suguru N. Kudoh; Takahisa Taguchi; Chie Hosokawa

doi:10.1117/12.2275026

18 April 2017 Molecular dynamics in an optical trap of glutamate receptors labeled with quantum-dots on living neurons

Tatsunori Kishimoto, Yasuyo Maezawa, Suguru N. Kudoh, Takahisa Taguchi, Chie Hosokawa

Proceedings Volume 10252, Optical Manipulation Conference; 1025208 (2017) https://doi.org/10.1117/12.2275026
Event: SPIE Technologies and Applications of Structured Light, 2017, Yokohama, Japan

Abstract

Molecular dynamics of glutamate receptor, which is major neurotransmitter receptor at excitatory synapse located on neuron, is essential for synaptic plasticity in the complex neuronal networks. Here we studied molecular dynamics in an optical trap of AMPA-type glutamate receptor (AMPAR) labeled with quantum-dot (QD) on living neuronal cells with fluorescence imaging and fluorescence correlation spectroscopy (FCS). When a 1064-nm laser beam for optical trapping was focused on QD-AMPARs located on neuronal cells, the fluorescence intensity of QD-AMPARs gradually increased at the focal spot. Using single-particle tracking of QD-AMPARs on neurons, the average diffusion coefficient decreased in an optical trap. Moreover, the decay time obtained from FCS analysis increased with the laser power and the initial assembling state of AMPARs depended on culturing day, suggesting that the motion of QD-AMPAR was constrained in an optical trap.

Citation Download Citation

Tatsunori Kishimoto, Yasuyo Maezawa, Suguru N. Kudoh, Takahisa Taguchi, and Chie Hosokawa "Molecular dynamics in an optical trap of glutamate receptors labeled with quantum-dots on living neurons", Proc. SPIE 10252, Optical Manipulation Conference, 1025208 (18 April 2017); https://doi.org/10.1117/12.2275026

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KEYWORDS

Neurons

Optical tweezers

Fluorescence correlation spectroscopy

Neurotransmitters

Diffusion

Laser induced fluorescence

Molecular lasers

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