Modelling Of 3-D Confocal Imaging At High Numerical Aperture In Fluorescence

H. T. M. van der Voort; G. J. Brakenhoff

doi:10.1117/12.950303

9 February 1989 Modelling Of 3-D Confocal Imaging At High Numerical Aperture In Fluorescence

H. T. M. van der Voort, G. J. Brakenhoff

Proceedings Volume 1028, Scanning Imaging; (1989) https://doi.org/10.1117/12.950303
Event: 1988 International Congress on Optical Science and Engineering, 1988, Hamburg, Germany

Abstract

The imaging properties of a model high aperture fluorescence confocal microscope are studied by way of numerical methods. The model consists of an aberration-free high aperture objective illuminated by a monochromatic linear or circular polarized light source, and a detection system equipped with a finite sized pinhole. The computation of the intensity distribution near the confocal plane is based on electromagnetic diffraction theory. We study the 3-D imaging properties of the model by examining the images of three different objects; points, lines and planes. We calculate the resolving power for these objects as a function of their orientation and the size of the detector pinhole. The results indicate the necessity for image analysis schemes to take these effects into account.

Citation Download Citation

H. T. M. van der Voort and G. J. Brakenhoff "Modelling Of 3-D Confocal Imaging At High Numerical Aperture In Fluorescence", Proc. SPIE 1028, Scanning Imaging, (9 February 1989); https://doi.org/10.1117/12.950303

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