Large scale serial two-photon microscopy to investigate local vascular changes in whole rodent brain models of Alzheimer's disease

P. Delafontaine-Martel; J. Lefebvre; R. Damseh; A. Castonguay; P. Tardif; F. Lesage

doi:10.1117/12.2290060

23 February 2018 Large scale serial two-photon microscopy to investigate local vascular changes in whole rodent brain models of Alzheimer's disease

P. Delafontaine-Martel, J. Lefebvre, R. Damseh, A. Castonguay, P. Tardif, F. Lesage

Author Affiliations +

Proceedings Volume 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII; 104982O (2018) https://doi.org/10.1117/12.2290060
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

In this study, an automated serial two-photon microscope was used to image a fluorescent gelatin filled rodent’s brain in 3D. A method to compute vascular density using automatic segmentation was combined with coregistration techniques to build group-level vasculature metrics. By studying the medial prefrontal cortex and the hippocampal formation of 3 age groups (2, 4.5 and 8 months old), we compared vascular density for both WT and an Alzheimer model transgenic brain (APP/PS1). We observe a loss of vascular density caused by the ageing process and we propose further analysis to confirm our results.

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P. Delafontaine-Martel, J. Lefebvre, R. Damseh, A. Castonguay, P. Tardif, and F. Lesage "Large scale serial two-photon microscopy to investigate local vascular changes in whole rodent brain models of Alzheimer's disease", Proc. SPIE 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII, 104982O (23 February 2018); https://doi.org/10.1117/12.2290060

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