Fluorescent molecular force probes for rheology and mechanobiology

Shohei Saito

doi:10.1117/12.2322936

24 April 2018 Fluorescent molecular force probes for rheology and mechanobiology

Shohei Saito

Proceedings Volume 10711, Biomedical Imaging and Sensing Conference; 107110J (2018) https://doi.org/10.1117/12.2322936
Event: SPIE Structured Light, 2018, Yokohama, Japan

Abstract

Fluorescent molecular force probes have been developed for rheology and mechanobiology. Single covalent bond is generally cleaved by nano-Newton force, which has been confirmed by the analyses of AFM and optical tweezers. To quantitatively evaluate stress concentration in stretched polymeric materials or biological systems before they break, pico- Newton force must be detected at molecular scale, and therefore molecular force probes based on a bond-breaking mechanism cannot be used for this purpose. Here we have explored flexible force probes that show fluorescence response by a conformational change of flapping π-conjugated molecules (FLAP), which have the potential to realize fluorescence response to pico-Newton forces. In this presentation, a series of FLAPs synthesized in our laboratory will be introduced in connection with the force mapping technology.

Citation Download Citation

Shohei Saito "Fluorescent molecular force probes for rheology and mechanobiology", Proc. SPIE 10711, Biomedical Imaging and Sensing Conference, 107110J (24 April 2018); https://doi.org/10.1117/12.2322936

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