Development of pulsed blue light technologies for bacterial biofilm disruption

Violet V. Bumah; Daniela Masson-Meyers; Dawn Castel; Chris Castel; Chukuka S. Enwemeka

doi:10.1117/12.2510699

7 March 2019 Development of pulsed blue light technologies for bacterial biofilm disruption

Violet V. Bumah, Daniela Masson-Meyers, Dawn Castel, Chris Castel, Chukuka S. Enwemeka

Proceedings Volume 10863, Photonic Diagnosis and Treatment of Infections and Inflammatory Diseases II; 108630U (2019) https://doi.org/10.1117/12.2510699
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

In recent studies, we showed that pulsed blue light is more potent in suppressing bacterial growth than continuous wave blue light. The potency of pulsed blue light makes it a viable antimicrobial for suppressing bacteria growth in biofilms, where the protective cover of the biofilm makes it is tougher to suppress bacteria. Consequently, we studied the efficacy of pulsed 450 nm light in suppressing the growth of MRSA and P. acnes biofilms. The results showed 100% bacterial suppression in planktonic cultures of MRSA irradiated with 7.6 J/cm² three times a day, using 3 mW/cm² irradiance, and in P. acnes planktonic cultures irradiated with 5 J/cm² thrice daily for 3 days, using 2 mW/cm² irradiance. However, a similar 100% suppression was not attained in MRSA or P. acnes biofilms irradiated thrice daily for 3 days at various fluences; but LIVE/DEAD assay showed a degree of bacterial suppression, with more live cells in controls than irradiated biofilms, and more dead cells in irradiated than control biofilms. In addition, while control biofilms had intact biofilm networks, irradiated biofilms had disrupted biofilm. The higher the dose, the more bacterial suppression and biofilm disruption. These findings confirm our previous reports that 100% bacterial suppression is attainable with pulsed blue light, and suggests further modification of the treatment protocol in order to achieve 100% bacterial suppression in biofilms.

Citation Download Citation

Violet V. Bumah, Daniela Masson-Meyers, Dawn Castel, Chris Castel, and Chukuka S. Enwemeka "Development of pulsed blue light technologies for bacterial biofilm disruption", Proc. SPIE 10863, Photonic Diagnosis and Treatment of Infections and Inflammatory Diseases II, 108630U (7 March 2019); https://doi.org/10.1117/12.2510699

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