The susceptibility of oral bacteria to antibacterial photodynamic therapy

Si-Mook Kang; Baek-Il Kim

doi:10.1117/12.2507675

7 March 2019 The susceptibility of oral bacteria to antibacterial photodynamic therapy

Si-Mook Kang, Baek-Il Kim

Proceedings Volume 10863, Photonic Diagnosis and Treatment of Infections and Inflammatory Diseases II; 108631B (2019) https://doi.org/10.1117/12.2507675
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

It has been found that the oral cavity and tongue are the second abundant habitats of bacteria in the body. The oral microbiome is essential to maintain oral health, while it also has the potential to cause disease due to having pathogens simultaneously. In this study, seven oral bacterial strains which are known to be associated with an oral disease were selected to evaluate the antibiotic susceptibility to four different photosensitizers (PSs). Stock solution (1 mg/mℓ) of four PSs was prepared by dissolving the powder in dimethyl sulfoxide for curcumin and protoporphyrin IX, and distilled water for resazurin and riboflavin. The inoculation of 20 μℓ of a 24 h cultured bacterial suspension mixed with 1,980 μℓ each test solution and then a light source was placed in front of the mixed solution. The LED excitation wavelength was 405 nm and its energy was 84.5 mW/cm² for 300 s. Bacterial viability was determined by measuring the absorbance at 655 nm with a microplate reader. It was found that all PSs affect the viability of some oral bacteria by irradiated with light. The antibacterial susceptibility on the different PSs and visible blue light irradiation were different depending on each bacterial strain. Moreover, the same bacterial strain showed different effects depending on the type of the PSs. From the above results, antibacterial photodynamic therapy composed of light source and photosensitizers can be used to control the oral bacteria strains which are related to oral disease.

Citation Download Citation

Si-Mook Kang and Baek-Il Kim "The susceptibility of oral bacteria to antibacterial photodynamic therapy", Proc. SPIE 10863, Photonic Diagnosis and Treatment of Infections and Inflammatory Diseases II, 108631B (7 March 2019); https://doi.org/10.1117/12.2507675

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available