A diffuse optical tomography system for whole-brain functional imaging in mice using multiple camera views (Conference Presentation)

Zachary E. Markow; Matthew D. Reisman; Adam Q. Bauer; Adam T. Eggebrecht; Mark A. Anastasio; Joseph P. Culver

doi:10.1117/12.2508997

4 March 2019 A diffuse optical tomography system for whole-brain functional imaging in mice using multiple camera views (Conference Presentation)

Zachary E. Markow, Matthew D. Reisman, Adam Q. Bauer, Adam T. Eggebrecht, Mark A. Anastasio, Joseph P. Culver

Author Affiliations +

Proceedings Volume 10874, Optical Tomography and Spectroscopy of Tissue XIII; 108741G (2019) https://doi.org/10.1117/12.2508997
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

For functional neuroimaging, existing small-animal diffuse optical tomography (DOT) systems either do not provide adequate temporal sampling rates, have sparse spatial sampling, or have limited three-dimensional fields of view. To achieve adequate frame rates (1-10 Hz), we have constructed a system using sCMOS detection-based DOT, with asymmetric measurements, with many (>10,000) detectors and fewer (<100) structured illumination patterns (using digital micromirror devices: DMDs). The system employs multiple views, involving multiple cameras and illuminators, to provide a three-dimensional field of view. To coregister the measurements with the mouse head anatomy, we developed a surface profiling method in which point illumination patterns are scanned over the mouse head and combined with calibration data to create three-dimensional point clouds and meshes representing the head. We applied this method to a 3D-printed figurine, and the resulting mesh had surface vertices whose positions deviated 0.4 ± 0.2 mm (mean ± SD) from the original "ground truth" mesh that had been employed to 3D-print the figurine. To evaluate the imaging system's resolution, field of view, and sensitivity versus depth, we placed simulated activations at different depths within a tissue model of a real mouse head imaged with our surface profiling method. Results indicate that this imaging system is sensitive to absorption changes at depths of >3 mm. In addition, a partial (one-camera, one-illuminator) version of the system successfully imaged neural activations evoked by forepaw stimulation of a live mouse.

Conference Presentation

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Zachary E. Markow, Matthew D. Reisman, Adam Q. Bauer, Adam T. Eggebrecht, Mark A. Anastasio, and Joseph P. Culver "A diffuse optical tomography system for whole-brain functional imaging in mice using multiple camera views (Conference Presentation)", Proc. SPIE 10874, Optical Tomography and Spectroscopy of Tissue XIII, 108741G (4 March 2019); https://doi.org/10.1117/12.2508997

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KEYWORDS

Imaging systems

Cameras

Diffuse optical tomography

Functional imaging

Head

3D metrology

Digital micromirror devices

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