Correlated simultaneous fluorescence and phosphorescence lifetime imaging for metabolic mapping and oxygen sensing in living cells

Sviatlana Kalinina; Patrick Schaefer; Bjorn von Einem; Angelika Rück

doi:10.1117/12.2513590

22 February 2019 Correlated simultaneous fluorescence and phosphorescence lifetime imaging for metabolic mapping and oxygen sensing in living cells

Sviatlana Kalinina, Patrick Schaefer, Bjorn von Einem, Angelika Rück

Author Affiliations +

Proceedings Volume 10882, Multiphoton Microscopy in the Biomedical Sciences XIX; 1088232 (2019) https://doi.org/10.1117/12.2513590
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

In combination with laser scanning microscopes, optical imaging technologies based on time correlated single photon counting (TCSPC) are successfully used in fluorescence lifetime imaging microscopy (FLIM) providing monitoring of intracellular intrinsic metabolic coenzymes as NAD(P)H (nicotinamide adenine dinucleotide (phosphate)). Due to oxygen-dependent quenching of the phosphorescence of some compounds including transition metal complexes, the phosphorescence lifetime imaging microscopy (PLIM) can be used for evaluation of oxygen partial pressure (pO2). Using a multi-channel FLIM/PLIM system, we were able to monitor pO2 by PLIM simultaneously with NAD(P)H by FLIM providing complex metabolic and redox imaging of living cells and tissues.

Citation Download Citation

Sviatlana Kalinina, Patrick Schaefer, Bjorn von Einem, and Angelika Rück "Correlated simultaneous fluorescence and phosphorescence lifetime imaging for metabolic mapping and oxygen sensing in living cells", Proc. SPIE 10882, Multiphoton Microscopy in the Biomedical Sciences XIX, 1088232 (22 February 2019); https://doi.org/10.1117/12.2513590

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