Dissolution of single upconversion nanoparticles dispersed in aqueous buffers (Conference Presentation)

Yves Mely; Oleksii Dukhno; Frédéric Przybilla; Thomas Hirsch

doi:10.1117/12.2511077

13 March 2019 Dissolution of single upconversion nanoparticles dispersed in aqueous buffers (Conference Presentation)

Yves Mely, Oleksii Dukhno, Frédéric Przybilla, Thomas Hirsch

Proceedings Volume 10884, Single Molecule Spectroscopy and Superresolution Imaging XII; 108840V (2019) https://doi.org/10.1117/12.2511077
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Single-particle luminescence microscopy is a powerful method to extract information on biological systems that is not accessible by ensemble-level methods. Upconversion nanoparticles (UCNPs) are highly suited for single-particle microscopy, as they provide stable, non-blinking luminescence, and avoid biological autofluorescence by their anti-Stokes emission. Recently, ensemble measurements of diluted aqueous dispersions of UCNPs have shown an instability of luminescence over time due to particle dissolution-related effects. This can be especially detrimental for single-particle experiments. However, this effect has never been estimated at the individual particle level. Here, the luminescence response of individual UCNPs in aqueous conditions is investigated by quantitative wide-field microscopy. The particles exhibit a rapid luminescence loss, accompanied by large changes in spectral response, leading to a considerable heterogeneity in their luminescence and band intensity ratio. Moreover, the dissolution-caused intensity loss is not correlated with initial particle intensity or band ratio, which makes it virtually unpredictable. These effects and the subsequent development of their heterogeneity can be largely slowed down by adding millimolar concentrations of sodium fluoride in the buffer. As a consequence, our data indicate that single molecule microscopy experiments employing UCNPs in aqueous environment should be performed in conditions that carefully prevent these effects.

Conference Presentation

Citation Download Citation

Yves Mely, Oleksii Dukhno, Frédéric Przybilla, and Thomas Hirsch "Dissolution of single upconversion nanoparticles dispersed in aqueous buffers (Conference Presentation)", Proc. SPIE 10884, Single Molecule Spectroscopy and Superresolution Imaging XII, 108840V (13 March 2019); https://doi.org/10.1117/12.2511077

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KEYWORDS

Luminescence

Nanoparticles

Upconversion

Microscopy

Atmospheric particles

Particles

Biological research

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