Ultraviolet hyperspectral interferometric microscopy

Francisco E. Robles

doi:10.1117/12.2511967

4 March 2019 Ultraviolet hyperspectral interferometric microscopy

Francisco E. Robles

Proceedings Volume 10888, Biophysics, Biology and Biophotonics IV: the Crossroads; 108880A (2019) https://doi.org/10.1117/12.2511967
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Ultraviolet (UV) spectroscopy is a powerful tool for quantitative biochemical analysis, but its application to molecular imaging and microscopy has been limited. Here we describe our recent work on ultraviolet hyperspectral interferometric (UHI) microscopy, which leverages coherent detection of optical fields to overcome significant challenges associated with UV spectroscopy when applied to molecular imaging. We demonstrate that this method enables quantitative spectral analysis of important endogenous biomolecules with subcellular spatial resolution and sensitivity to nanometer-scaled structures for label-free molecular imaging of live cells.

Citation Download Citation

Francisco E. Robles "Ultraviolet hyperspectral interferometric microscopy", Proc. SPIE 10888, Biophysics, Biology and Biophotonics IV: the Crossroads, 108880A (4 March 2019); https://doi.org/10.1117/12.2511967

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