Graphene oxide based FRET probe for mast cell degranulation

Shuzhen Tang; Jianshu Xu; Yating Lin; Mengmeng Zheng; Yamin Lin; Yimei Huang; Shusen Xie; Juqiang Lin

doi:10.1117/12.2537508

20 November 2019 Graphene oxide based FRET probe for mast cell degranulation

Shuzhen Tang, Jianshu Xu, Yating Lin, Mengmeng Zheng, Yamin Lin, Yimei Huang, Shusen Xie, Juqiang Lin

Proceedings Volume 11190, Optics in Health Care and Biomedical Optics IX; 111902J (2019) https://doi.org/10.1117/12.2537508
Event: SPIE/COS Photonics Asia, 2019, Hangzhou, China

Abstract

Degranulation in mast cell is usually characterized by the release of tryptase. We developed a fluorescence resonance energy transfer (FRET) probe based on graphene oxide (GO) to detect tryptase released from mast cells. The GO based FRET probe is composed of GO and a self-assembled complex of tryptase-specific recognition peptide chains labeled with isothiocyanate fluorescein. The fluorescence intensity around the mast cells increased when the mast cells were stimulated with C48/80, a kind of reagent promoting degranulation. The fluorescence distribution is inhomogenous. The fluorescence intensity was dependent on the concentration of C48/80 and the stimulation time. These results demonstrate that GO-base FRET probe could be used to study degranulation in mast cells.

Citation Download Citation

Shuzhen Tang, Jianshu Xu, Yating Lin, Mengmeng Zheng, Yamin Lin, Yimei Huang, Shusen Xie, and Juqiang Lin "Graphene oxide based FRET probe for mast cell degranulation", Proc. SPIE 11190, Optics in Health Care and Biomedical Optics IX, 111902J (20 November 2019); https://doi.org/10.1117/12.2537508

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