A fast reconstruction method for super-resolution localization microscopy with gOMP

Hehe Ma; Yuexia Shu; Xin Liu

doi:10.1117/12.2538644

20 November 2019 A fast reconstruction method for super-resolution localization microscopy with gOMP

Hehe Ma, Yuexia Shu, Xin Liu

Proceedings Volume 11190, Optics in Health Care and Biomedical Optics IX; 111903K (2019) https://doi.org/10.1117/12.2538644
Event: SPIE/COS Photonics Asia, 2019, Hangzhou, China

Abstract

Super-resolution localization microscopy (SRLM) breaks the diffraction limit, making possible the observation of sub-cellular structures. Challenges remain in SRLM due to a long data acquisition time. To overcome the limitation, the methods based on compressed sensing (CS) have been proposed. However, at the current stage, the widely used sparsity-based localization methods, e.g., interior point method (IPM), is computationally intensive. To address the problem, in this paper, we introduce an alternative CS reconstruction method to super-resolution imaging model, which is achieved by using gOMP (generalized Orthogonal Matching Pursuit). A series of numerical simulations with varying emitter densities and signal-to-noise rations (SNRs) are performed to evaluate the performance of gOMP method. The results show that whatever gOMP or IPM is used in SRLM, the obtained localization accuracy is similar. But, the data-processing time of gOMP can be significantly reduced (< 100 times) than the previous reported IPM method. As a result, gOMP provides the potential for reducing the computational cost while maintaining a desired spatial resolution, which is beneficial for SRLM imaging.

Citation Download Citation

Hehe Ma, Yuexia Shu, and Xin Liu "A fast reconstruction method for super-resolution localization microscopy with gOMP", Proc. SPIE 11190, Optics in Health Care and Biomedical Optics IX, 111903K (20 November 2019); https://doi.org/10.1117/12.2538644

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