Photodynamic diagnosis with 5-aminolevulinic acid for intramucosal gastric cancer using green light

Daisuke Ihara; Hisanao Hazama; Takahiro Nishimura; Kunio Awazu

doi:10.1117/12.2545653

19 February 2020 Photodynamic diagnosis with 5-aminolevulinic acid for intramucosal gastric cancer using green light

Daisuke Ihara, Hisanao Hazama, Takahiro Nishimura, Kunio Awazu

Proceedings Volume 11220, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXIX; 112200P (2020) https://doi.org/10.1117/12.2545653
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Photodynamic diagnosis (PDD) of gastric cancer with protoporphyrin IX (PpIX) induced by 5-aminolevulinic acid (ALA) will lead to a method to estimate the invasive depth. The excitation light for ALA-based PDD is attenuated by absorption and scattering of the mucosal tissue according to its wavelength and the difference contains the fluorophore depth information. To realize the method to estimate the invasive depth for the intramucosal cancer, this study investigated the differences of the attenuation rate between two excitation wavelengths. Considering the optical properties of mucosa and PpIX, relationships between the invasive depths of cancer containing PpIX and the fluorescence intensity ratios detected from the mucosa were estimated using a numerical model. An experiment was also performed using a pellet made of epoxy resin containing PpIX. The excitation lights with wavelengths of 405 and 505 nm having the same power density were each used to irradiate the pellet placed at the fluorophore depth of 0 mm, 1.0 mm, and 1.7 mm from the mucosal surface to obtain fluorescence images, and the average fluorescence intensities of the images were evaluated. The numerical and experimental results suggested the possibility to estimate the fluorophore depth of the PpIX fluorescence source by comparing the fluorescence images with excitation lights at wavelengths of 405 and 505 nm.

Citation Download Citation

Daisuke Ihara, Hisanao Hazama, Takahiro Nishimura, and Kunio Awazu "Photodynamic diagnosis with 5-aminolevulinic acid for intramucosal gastric cancer using green light", Proc. SPIE 11220, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXIX, 112200P (19 February 2020); https://doi.org/10.1117/12.2545653

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
7 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Luminescence

Absorption

Cancer

Scattering

Tissues

Light scattering

Tissue optics

Keywords/Phrases

Search In:

Publication Years