Discrimination of brain tumor regional samples using combined visible light optical coherence microscopy and fluorescence imaging (Conference Presentation)

Johanna Gesperger; Antonia Lichtenegger; Thomas Roetzer; Pablo Eugui Arrizabalaga; Barbara Kiesel; Danielle Jade Harper; Conrad Wiliam Merkle; Marco Augustin; Georg Widhalm; Adelheid Woehrer; Bernhard Baumann

doi:10.1117/12.2544652

9 March 2020 Discrimination of brain tumor regional samples using combined visible light optical coherence microscopy and fluorescence imaging (Conference Presentation)

Johanna Gesperger, Antonia Lichtenegger, Thomas Roetzer, Pablo Eugui Arrizabalaga, Barbara Kiesel, Danielle Jade Harper, Conrad Wiliam Merkle, Marco Augustin, Georg Widhalm, Adelheid Woehrer, Bernhard Baumann

Author Affiliations +

Proceedings Volume 11226, Neural Imaging and Sensing 2020; 112261G (2020) https://doi.org/10.1117/12.2544652
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Maximal safe resection is of utmost importance when operating on intracranial tumors. However, the discrimination between physiological and malignant tissue is challenging. A solution is 5-aminolevulinic acid (5-ALA) induced protoporphyrin IX (PPIX) fluorescence of malignant cells, which has evolved as powerful intraoperative tool for tumor identification. We investigated 43 samples obtained during 5-ALA guided surgery using a combined optical coherence microscopy (OCM) and fluorescence imaging system and compared results to cell density maps utilizing digital pathology. Based on fluorescence intensity, attenuation coefficients, and cell density, tumor core was differentiated from tumor-associated brain parenchyma with high sensitivity and specificity.

Conference Presentation

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Johanna Gesperger, Antonia Lichtenegger, Thomas Roetzer, Pablo Eugui Arrizabalaga, Barbara Kiesel, Danielle Jade Harper, Conrad Wiliam Merkle, Marco Augustin, Georg Widhalm, Adelheid Woehrer, and Bernhard Baumann "Discrimination of brain tumor regional samples using combined visible light optical coherence microscopy and fluorescence imaging (Conference Presentation)", Proc. SPIE 11226, Neural Imaging and Sensing 2020, 112261G (9 March 2020); https://doi.org/10.1117/12.2544652

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