A spectral de-mixing model for triplex in vivo imaging of optical coherence tomography contrast agents

Edwin Yuan; Peng Si; Saba Shevidi; Adam de la Zerda

doi:10.1117/12.2545501

21 February 2020 A spectral de-mixing model for triplex in vivo imaging of optical coherence tomography contrast agents

Edwin Yuan, Peng Si, Saba Shevidi, Adam de la Zerda

Proceedings Volume 11228, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXIV; 1122826 (2020) https://doi.org/10.1117/12.2545501
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

The ability to detect multiple contrast agents simultaneously would greatly enhance Optical Coherence Tomography (OCT) images, providing nuanced biological context to physiological structures. However, previous OCT contrast agent work has been limited to scenarios where only a single contrast agent could be robustly detected within each voxel. We present a novel spectroscopic technique for de-mixing the spectral signal from multiple OCT contrast agents within a single voxel. We validate our technique in vitro and also demonstrate in vivo imaging of three spectrally distinct gold nanobipyramids, trafficking within the lymphatic system of a live mouse. This approach opens the door to a much broader range of pre-clinical and clinical OCT applications where multiplexed labeling is desirable.

Conference Presentation

Citation Download Citation

Edwin Yuan, Peng Si, Saba Shevidi, and Adam de la Zerda "A spectral de-mixing model for triplex in vivo imaging of optical coherence tomography contrast agents", Proc. SPIE 11228, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXIV, 1122826 (21 February 2020); https://doi.org/10.1117/12.2545501

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