Optimization of optical clearing and large-scale 3D imaging of whole intact murine lung (Conference Presentation)

Lorenzo F. Ochoa

doi:10.1117/12.2551579

9 March 2020 Optimization of optical clearing and large-scale 3D imaging of whole intact murine lung (Conference Presentation)

Lorenzo F. Ochoa

Author Affiliations +

Proceedings Volume 11231, Design and Quality for Biomedical Technologies XIII; 1123109 (2020) https://doi.org/10.1117/12.2551579
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Numerous pulmonary disorders are associated with fibrosis, however, current methodology falls short when studying turbid tissue. Optical clearing (OC) offers a chemical-based approach for deep tissue imaging. We optimized organ-level OC approach and combined it with large-scale, label-free multiphoton microscopy (MPM) and second harmonic generation microscopy (SHGM) to reveal fibrillar collagen in whole murine lungs. The standardization of several underlying steps allowed for a streamline approach. This method revealed significant differences in collagen deposition between control and treated lungs, while establishing a new approach based on OC and MPM/SHGM imaging for 3D analysis of lung fibrosis in the whole lung.

Conference Presentation

Citation Download Citation

Lorenzo F. Ochoa "Optimization of optical clearing and large-scale 3D imaging of whole intact murine lung (Conference Presentation)", Proc. SPIE 11231, Design and Quality for Biomedical Technologies XIII, 1123109 (9 March 2020); https://doi.org/10.1117/12.2551579

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