In vivo cuticle intact Drosophila mushroom body imaging using laser scanning optical resolution photoacoustic microscopy (Conference Presentation)

Kai-Yao Chang; Wen-Hung Shih; Shun-Chi Wu; Kwok Ho Lam; Yen-Yin Lin; Ann-Shyn Chiang; Meng-Lin Li

doi:10.1117/12.2547187

6 March 2020 In vivo cuticle intact Drosophila mushroom body imaging using laser scanning optical resolution photoacoustic microscopy (Conference Presentation)

Kai-Yao Chang, Wen-Hung Shih, Shun-Chi Wu, Kwok Ho Lam, Yen-Yin Lin, Ann-Shyn Chiang, Meng-Lin Li

Author Affiliations +

Proceedings Volume 11240, Photons Plus Ultrasound: Imaging and Sensing 2020; 112401Z (2020) https://doi.org/10.1117/12.2547187
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Surgical removal of the head cuticle of Drosophila is necessary for optical brain imaging, leading to the Drosophila death in a short period of time and thus hindering long term monitoring. Targeting to the unmet need of surgery free procedure for Drosophila brain researches, in this study, laser scanning optical resolution photoacoustic microscopy (LSOR-PAM) of in vivo cuticle intact RCaMP labeled Drosophila mushroom body (MB) is presented. The MB is a higher-order olfactory center in Drosophila brain. This study paves the way toward exploring LSOR-PAM functional imaging capability of cuticle intact Drosophila brain under olfactory stimulus.

Conference Presentation

Citation Download Citation

Kai-Yao Chang, Wen-Hung Shih, Shun-Chi Wu, Kwok Ho Lam, Yen-Yin Lin, Ann-Shyn Chiang, and Meng-Lin Li "In vivo cuticle intact Drosophila mushroom body imaging using laser scanning optical resolution photoacoustic microscopy (Conference Presentation)", Proc. SPIE 11240, Photons Plus Ultrasound: Imaging and Sensing 2020, 112401Z (6 March 2020); https://doi.org/10.1117/12.2547187

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available