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9 March 2020 FLIm and Raman imaging for detecting micro-environmental changes in bovine pericardium upon genipin cross-linking (Conference Presentation)
Tanveer Ahmed Shaik, Alba Alfonso García, Martin Richter, Florian Korinth, Anne K. Haudenschild, James F. Mcmasters, Christoph Krafft, Jürgen Popp, Laura Marcu
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Proceedings Volume 11243, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII; 112431B (2020) https://doi.org/10.1117/12.2544225
Event: SPIE BiOS, 2020, San Francisco, California, United States
Abstract
Genipin cross-linked engineered tissues are 10000 times less toxic than glutaraldehyde cross-linked tissues. Hence, genipin is a better fixative to support the recellularization of tissue-engineered constructs such as vascular grafts. Here, we demonstrate the ability of fiber-based Fluorescence Lifetime Imaging (FLIm) guided Raman spectroscopy to monitor the quality of genipin cross-linked vascular grafts with high speed and specificity. Current results indicate that the fluorescence lifetime of AR-BP shortens upon GE cross-linking. Raman spectroscopy reveals secondary structural changes occurring in the extracellular matrix of pericardia that correspond to Amide I, Amide III and C-C stretch vibrations. We conclude that FLIm guided Raman imaging can detect cross-linking signatures with biochemical specificity and that this imaging modality provides a non-destructive and label-free method to assess the quality of vascular grafts
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Tanveer Ahmed Shaik, Alba Alfonso García, Martin Richter, Florian Korinth, Anne K. Haudenschild, James F. Mcmasters, Christoph Krafft, Jürgen Popp, and Laura Marcu "FLIm and Raman imaging for detecting micro-environmental changes in bovine pericardium upon genipin cross-linking (Conference Presentation)", Proc. SPIE 11243, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII, 112431B (9 March 2020); https://doi.org/10.1117/12.2544225
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KEYWORDS
Raman spectroscopy
Analog electronics
Data acquisition
Fluorescence lifetime imaging
In vitro testing
Luminescence
Nondestructive evaluation
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