Frequency-tunable two-color ultrafast fiber laser for nonlinear excitation of NADH and FAD

Axel Friedenauer; Konrad Birkmeier; Andreas Brodschelm; Max Eisele; Christoph Skrobol; Patrick Leisching

doi:10.1117/12.2546120

14 February 2020 Frequency-tunable two-color ultrafast fiber laser for nonlinear excitation of NADH and FAD

Axel Friedenauer, Konrad Birkmeier, Andreas Brodschelm, Max Eisele, Christoph Skrobol, Patrick Leisching

Proceedings Volume 11244, Multiphoton Microscopy in the Biomedical Sciences XX; 112441M (2020) https://doi.org/10.1117/12.2546120
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

We will present our latest innovations about ultrafast fiber lasers and show how multiphoton microscopy can benefit from these developments. Wavelengths around 900 nm and pulse durations as short as 100 fs remain a challenge for fiber lasers. Here we present a two-color femtosecond fiber laser system with synchronous outputs. One arm emits pulses at a central wavelength of 780 nm and the novel second laser arm is continuously tunable in its central wavelength between 810 nm and 950 nm. This allows the independent excitation of NADH and FAD and therefore enables optical metabolism and oxygen imaging of cells via FLIM and PLIM measurements.

Citation Download Citation

Axel Friedenauer, Konrad Birkmeier, Andreas Brodschelm, Max Eisele, Christoph Skrobol, and Patrick Leisching "Frequency-tunable two-color ultrafast fiber laser for nonlinear excitation of NADH and FAD", Proc. SPIE 11244, Multiphoton Microscopy in the Biomedical Sciences XX, 112441M (14 February 2020); https://doi.org/10.1117/12.2546120

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