Nanofluidic label-free single biomolecule detection (Conference Presentation)

Barbora Spackova; Johan Tenghamn; Joachim Fritzsche; Christoph Langhammer

doi:10.1117/12.2544736

10 March 2020 Nanofluidic label-free single biomolecule detection (Conference Presentation)

Barbora Spackova, Johan Tenghamn, Joachim Fritzsche, Christoph Langhammer

Proceedings Volume 11254, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVII; 112540O (2020) https://doi.org/10.1117/12.2544736
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

We present a nanofluidic optical device based on light-scattering microscopy, which enables label-free detection and quantitative analysis of individual biomolecules freely moving in solution. The key component is a nanochannel with subwavelength dimensions, which is imaged by dark-field microscopy. Due to interference between light scattered by the nanochannel and a biomolecule inside it, a single biomolecule can be directly detected. In addition, the molecular weight can be measured, both by tracking the Brownian motion and from the optical contrast. This is demonstrated for both single DNAs and proteins with molecular weights ranging down to tens of kDa.

Conference Presentation

Citation Download Citation

Barbora Spackova, Johan Tenghamn, Joachim Fritzsche, and Christoph Langhammer "Nanofluidic label-free single biomolecule detection (Conference Presentation)", Proc. SPIE 11254, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVII, 112540O (10 March 2020); https://doi.org/10.1117/12.2544736

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