A Video Rate Confocal Laser Beam Scanning Light Microscope Using An Image Dissector

Seth R. Goldstein; Thomas Hubin; Scott Rosenthal; Clayton Washburn

doi:10.1117/12.962709

22 December 1989 A Video Rate Confocal Laser Beam Scanning Light Microscope Using An Image Dissector

Seth R. Goldstein, Thomas Hubin, Scott Rosenthal, Clayton Washburn

Proceedings Volume 1161, New Methods in Microscopy and Low Light Imaging; (1989) https://doi.org/10.1117/12.962709
Event: 33rd Annual Technical Symposium, 1989, San Diego, United States

Abstract

A video rate confocal reflected light microscope with no moving parts has been developed. Return light from an acousto-optically raster scanned laser beam is imaged from the microscope stage onto the photocathode of an Image Dissector Tube (IDT). Confocal operation is achieved by appropriately raster scanning with the IDT x and y deflection coils so as to continuously "sample" that portion of the photocathode that is being instantaneously illuminated by the return image of the scanning laser spot. Optimum IDT scan parameters and geometric distortion correction parameters are determined under computer control within seconds and are then continuously applied to insure system alignment. The system is operational and reflected light images from a variety of objects have been obtained. The operating principle can be extended to fluorescence and transmission microscopy.

Citation Download Citation

Seth R. Goldstein, Thomas Hubin, Scott Rosenthal, and Clayton Washburn "A Video Rate Confocal Laser Beam Scanning Light Microscope Using An Image Dissector", Proc. SPIE 1161, New Methods in Microscopy and Low Light Imaging, (22 December 1989); https://doi.org/10.1117/12.962709

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