Nanometer Measurements Of Motor Protein And Membrane Glycoprotein Movements

M. P. Sheetz; J. Gelles; E. R. Steuer; B. J. Schnapp; J. Dubinska; H. Qian; E. Elson

doi:10.1117/12.962707

22 December 1989 Nanometer Measurements Of Motor Protein And Membrane Glycoprotein Movements

M. P. Sheetz, J. Gelles, E. R. Steuer, B. J. Schnapp, J. Dubinska, H. Qian, E. Elson

Proceedings Volume 1161, New Methods in Microscopy and Low Light Imaging; (1989) https://doi.org/10.1117/12.962707
Event: 33rd Annual Technical Symposium, 1989, San Diego, United States

Abstract

The recent application of digital imaging technologies to the analysis of video images from a DIC (differential interference contrast) light microscope has given 1-2 nm precision for the measurement of movements of submicron particles at 30 Hz. [Gelles et al., Nature 331(1988)450]. This technique has been applied to the study of the movements of 1) motor proteins (kinesin and cytoplasmic dynein) on microtubules (MTs) and 2) membrane glycoproteins in cellular plasma membranes. In both cases the nanometer measurements have provided important new insights into the molecular mechanisms which govern those biological movements.

Citation Download Citation

M. P. Sheetz, J. Gelles, E. R. Steuer, B. J. Schnapp, J. Dubinska, H. Qian, and E. Elson "Nanometer Measurements Of Motor Protein And Membrane Glycoprotein Movements", Proc. SPIE 1161, New Methods in Microscopy and Low Light Imaging, (22 December 1989); https://doi.org/10.1117/12.962707

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