Designing a multimodal imaging workflow for large area fluorescence with nonlinear optical microscopy for oral neoplasia detection: preclinical model and clinical sample study

Rahul Pal; Paula Villarreal; Xiaoying Yu; Suimin Qiu; Orly Coblens; Gracie Vargas

doi:10.1117/12.2591286

5 March 2021 Designing a multimodal imaging workflow for large area fluorescence with nonlinear optical microscopy for oral neoplasia detection: preclinical model and clinical sample study

Rahul Pal, Paula Villarreal, Xiaoying Yu, Suimin Qiu, Orly Coblens, Gracie Vargas

Author Affiliations +

Proceedings Volume 11622, Multiscale Imaging and Spectroscopy II; 116220I (2021) https://doi.org/10.1117/12.2591286
Event: SPIE BiOS, 2021, Online Only

Abstract

The recognized need to develop better clinical approaches for detection of epithelial cancers and potentially malignant lesions than currently used has motivated work in development of noninvasive fluorescence imaging devices. While individual large area imaging and microscopic techniques are promising, recent trends have explored combinations that could merge strengths. The study will discuss a workflow to combine strengths of label-free nonlinear optical microscopy (NLOM) which has shown promise for optical biopsy but is limited in scannable area with widefield autofluorescence microscopy providing large surface area assessment, in studies conducted in both a hamster model for oral neoplasia and inflammation and in surgical oral cancer specimens.

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Rahul Pal, Paula Villarreal, Xiaoying Yu, Suimin Qiu, Orly Coblens, and Gracie Vargas "Designing a multimodal imaging workflow for large area fluorescence with nonlinear optical microscopy for oral neoplasia detection: preclinical model and clinical sample study", Proc. SPIE 11622, Multiscale Imaging and Spectroscopy II, 116220I (5 March 2021); https://doi.org/10.1117/12.2591286

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