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The detection sensitivity of optoacoustic spectroscopy at the short-wavelength infrared (SWIR) region is reduced by water absorption in aqueous solutions. Our work reports marked improvements in the sensitivity of optoacoustic spectroscopic measurements of proteins, lipids, and glucose when performed at 4 °C, compared to conventional optoacoustic or IR spectroscopy. Studying the effect of water temperature on optoacoustic signals revealed a polarity change at temperatures below 4 °C (muting temperature). The dependence of the optoacoustic signal and the muting temperature on sample concentration were further investigated, demonstrating that changes in these dependencies enable accurate quantification of the solute concentration.
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Jaya Prakash, Mir Mehdi Seyedebrahimi, Ara Ghazaryan, Jaber Malekzadeh-Najafabadi, Vipul Gujrati, Vasilis Ntziachristos, "Cooled infrared optoacoustic spectroscopy (CIROAS) for accurate sensing based on water muting," Proc. SPIE 11642, Photons Plus Ultrasound: Imaging and Sensing 2021, 116422B (7 March 2021); https://doi.org/10.1117/12.2582335