Viral nucleic acid detection with CRISPR-Cas12a using high contrast cleavage detection on micro-ring resonator biosensors

Li Liu; Michael Dubrovsky; Sarat Gundavarapu; Diedrik Vermeulen; Ke Du

doi:10.1117/12.2580053

5 March 2021 Viral nucleic acid detection with CRISPR-Cas12a using high contrast cleavage detection on micro-ring resonator biosensors

Li Liu, Michael Dubrovsky, Sarat Gundavarapu, Diedrik Vermeulen, Ke Du

Author Affiliations +

Proceedings Volume 11662, Frontiers in Biological Detection: From Nanosensors to Systems XIII; 1166207 (2021) https://doi.org/10.1117/12.2580053
Event: SPIE BiOS, 2021, Online Only

Abstract

Micro-ring resonators have emerged as a powerful platform for analyzing and detecting biomolecules at low concentrations. Here we demonstrate a high contrast cleavage detection (HCCD) assay on a micro-ring resonator to sense the cleavage of DNA reporterslinked to high-contrast nanoparticles (NPs), leading to dramatic optical signal amplification. The HCCD mechanism is coupled with a CRISPR-Cas12a assay for rapid and sensitive on-chip nucleic acid detection. Leveraging high-contrast gold nanoparticle (AuNP) reporters, an ~8 nm resonance shift is observed by using a 1 nM of complementary DNA (cDNA) target, matching part of the SARS-CoV-2 sequence. In addition, we show that a micro-ring resonator can not only record the entire surface functionalization process, as has been show previously, but also monitor CRISPR reactions in-situ. This work is the first step toward novel nucleic acid amplification-free detection via a combination of integrated photonics and CRISPR-Cas collateral cleavage assays.

Conference Presentation

Citation Download Citation

Li Liu, Michael Dubrovsky, Sarat Gundavarapu, Diedrik Vermeulen, and Ke Du "Viral nucleic acid detection with CRISPR-Cas12a using high contrast cleavage detection on micro-ring resonator biosensors", Proc. SPIE 11662, Frontiers in Biological Detection: From Nanosensors to Systems XIII, 1166207 (5 March 2021); https://doi.org/10.1117/12.2580053

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available