Isotropic divide-stages-to process convolutional neural network enhanced double-ring modulated SPIM microscopy (IDDR-SPIM): achieving 5D super-resolution imaging in live cell

Yuxuan Zhao; Yao Zhou; Longbiao Chen; Peng Wang; Peng Fei

doi:10.1117/12.2601421

9 October 2021 Isotropic divide-stages-to process convolutional neural network enhanced double-ring modulated SPIM microscopy (IDDR-SPIM): achieving 5D super-resolution imaging in live cell

Yuxuan Zhao, Yao Zhou, Longbiao Chen, Peng Wang, Peng Fei

Author Affiliations +

Proceedings Volume 11900, Optics in Health Care and Biomedical Optics XI; 119002E (2021) https://doi.org/10.1117/12.2601421
Event: SPIE/COS Photonics Asia, 2021, Nantong, JS, China

Conference Poster

Abstract

The tradeoff between spatial-temporal resolutions and phototoxicity prevents fast 3D super-resolution imaging from living cells, hindering the reveal of the subcellular interactions. Key challenges lie in the quests to optimal excitation and efficient super-resolution. Here, we present a double-ring modulated SPIM design toward an ultrathin uniform light sheet with low side lobes. The combination of IDDR SPIM with our isotropic divide-stages-to-process (ID) networks allows live-cell imaging at isotropic 100 nm resolution with a volume rate up to 17 Hz for thousands of time points.

Citation Download Citation

Yuxuan Zhao, Yao Zhou, Longbiao Chen, Peng Wang, and Peng Fei "Isotropic divide-stages-to process convolutional neural network enhanced double-ring modulated SPIM microscopy (IDDR-SPIM): achieving 5D super-resolution imaging in live cell", Proc. SPIE 11900, Optics in Health Care and Biomedical Optics XI, 119002E (9 October 2021); https://doi.org/10.1117/12.2601421

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