Single-cell sorting of marine plankton based on micro-optical tweezers

Caiyun Miao; Jia Yu; Lingyan Kan; Huiping Liu; Yuandong Li

doi:10.1117/12.2601557

9 October 2021 Single-cell sorting of marine plankton based on micro-optical tweezers

Caiyun Miao, Jia Yu, Lingyan Kan, Huiping Liu, Yuandong Li

Proceedings Volume 11900, Optics in Health Care and Biomedical Optics XI; 119002J (2021) https://doi.org/10.1117/12.2601557
Event: SPIE/COS Photonics Asia, 2021, Nantong, JS, China

Conference Poster

Abstract

Marine plankton are micro and diverse, and most of them are still non-cultivable. So single-cell analysis, focused on the study of diversity and heterogeneity of their cells, has become a hot issue in marine microbiology research, which relies on sound single-cell preparation technology for upstream operations. By combining micro-optical tweezers technology with microfluidic droplet wrapping technology, we developed a fluorescence microscopic optical tweezers system for plankton sorting. Our system consists of a micro-optical tweezers module, a fluorescence module, an imaging module and a sorting module. Using this system, we conduct single-cell observation, monitoring, capture manipulation, separation and extraction of microplankton in seawater samples. The specific process is as follows: 1. Prepare samples, including grading filtration and enrichment of seawater sampled in Qingdao offshore. 2. Observe and select the target single-cell with a specific sorting chip on the microscopic optical tweezers single-cell sorting system. And perform visualized microdroplet single-cell sorting through optical tweezers capture manipulation. 3. Take out the target living single-cell after forming microdroplets. Using our experimental system, we are able to provide precise technical support for downstream experiments including single-cell culture, sequencing and monoclonality. In addition, our method has the advantages of flexible operation, intact cells and visualization as "what you see is what you get". Meanwhile, it has a 100% single-cell acquisition rate while maintaining the high viability of the sorted cell.

Citation Download Citation

Caiyun Miao, Jia Yu, Lingyan Kan, Huiping Liu, and Yuandong Li "Single-cell sorting of marine plankton based on micro-optical tweezers", Proc. SPIE 11900, Optics in Health Care and Biomedical Optics XI, 119002J (9 October 2021); https://doi.org/10.1117/12.2601557

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