Paper
7 December 2021 Ultra-broadband few-cycle laser pulses for simultaneous multi-color fluorescence microscopy applications via the SyncRGB-FLIM method
Christian Maibohm, Rodrigo Ferreira, Oscar F. Silvestre, Rosa Romero, Helder Crespo, Jana B. Nieder
Author Affiliations +
Abstract
Ultra-broadband lasers allow simultaneous excitation of various dyes and endogenous markers, used for metabolic imaging during treatment of live-cells with anticancer drugs. The versatility of few cycle lasers for biomedical imaging is demonstrated.
© (2021) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Christian Maibohm, Rodrigo Ferreira, Oscar F. Silvestre, Rosa Romero, Helder Crespo, and Jana B. Nieder "Ultra-broadband few-cycle laser pulses for simultaneous multi-color fluorescence microscopy applications via the SyncRGB-FLIM method", Proc. SPIE 11922, Advances in Microscopic Imaging III, 119220G (7 December 2021); https://doi.org/10.1117/12.2615698
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KEYWORDS
Microscopy

Luminescence

Femtosecond phenomena

Laser sources

Chromophores

Laser applications

Fluorescence lifetime imaging

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