Dual-labeled anti-CEA antibody for near-infrared fluorescence and PET imaging of colorectal cancer

Paul J. Yazaki; Lin Li; Megan Minnix; Anakim Sherman; Teresa Hong; Tove Olafsen; Erasmus Poku; Yuman Fong; John E. Shively

doi:10.1117/12.2626239

4 March 2022 Dual-labeled anti-CEA antibody for near-infrared fluorescence and PET imaging of colorectal cancer

Paul J. Yazaki, Lin Li, Megan Minnix, Anakim Sherman, Teresa Hong, Tove Olafsen, Erasmus Poku, Yuman Fong, John E. Shively

Author Affiliations +

Proceedings Volume 11943, Molecular-Guided Surgery: Molecules, Devices, and Applications VIII; 1194307 (2022) https://doi.org/10.1117/12.2626239
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Background/purpose. Multi-modality imaging is a major diagnostic component for cancer management, enabling detection, staging of disease, targeting therapy, and monitoring the therapeutic response. The development of a single agent for real-time non-invasive immunoPET imaging and fluorescence guided surgery (FGS) will provide the next generation tool in the surgical management of cancer. Methods. The humanized anti-CEA M5A-IR800 “sidewinder” (M5A-IR800-SW) antibody-dye conjugate has a NIR 800nm dye incorporated into a PEGylated linker and conjugated with the metal chelate p-SCN-Bn-deferoxamine (DFO) for zirconium-89 (⁸⁹Zr, half-life 78.4 h) PET imaging. The dual-labeled ⁸⁹Zr-DFO-M5A-SW-IR800 was evaluated for NIR fluorescence imaging, PET/MRI imaging, terminal tissue biodistribution and blood clearance in a human colorectal cancer LS174T xenograft mouse model. Results. The ⁸⁹Zr-DFO-M5A-SW-IR800 NIR fluorescence imaging showed high tumor and liver localization that was confirmed by PET imaging. Serial PET/MRI imaging was performed at 24 h, 48 h and 72 h and showed tumor localization visible at 24 h that persisted throughout the experiment. However, the PET scans showed higher activity for the liver than the tumor, compared to the NIR fluorescence imaging. This difference is an important finding as it quantifies the expected difference due to the sensitivity and depth of penetration between the 2 modalities.

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Paul J. Yazaki, Lin Li, Megan Minnix, Anakim Sherman, Teresa Hong, Tove Olafsen, Erasmus Poku, Yuman Fong, and John E. Shively "Dual-labeled anti-CEA antibody for near-infrared fluorescence and PET imaging of colorectal cancer", Proc. SPIE 11943, Molecular-Guided Surgery: Molecules, Devices, and Applications VIII, 1194307 (4 March 2022); https://doi.org/10.1117/12.2626239

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