Phosphorescence Lifetime Imaging (PLIM) with a multimodal multiphoton tomograph

K. König; H. G. Breunig

doi:10.1117/12.2607590

3 March 2022 Phosphorescence Lifetime Imaging (PLIM) with a multimodal multiphoton tomograph

K. König, H. G. Breunig

Proceedings Volume 11965, Multiphoton Microscopy in the Biomedical Sciences XXII; 1196504 (2022) https://doi.org/10.1117/12.2607590
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

We report on the use of a modified multimodal multiphoton CARS tomograph for time-resolved auto-fluorescence (FLIM) and auto-phosphorescence imaging (PLIM) of biological tissues. Time-correlated single photon counting (TCSPC) was applied to measure fluorescence and phosphorescence decay curves per pixel. For PLIM, the repetition rate of the 80 MHz tunable titanium:sapphire femtosecond laser was reduced by an acousto-optic modulator. We present FLIM and PLIM data of non-labelled bones after heating. The phosphorescence behavior shows a strong dependence on the heat treatment. This is of special interest for forensic and archeological research.

Conference Presentation

Citation Download Citation

K. König and H. G. Breunig "Phosphorescence Lifetime Imaging (PLIM) with a multimodal multiphoton tomograph", Proc. SPIE 11965, Multiphoton Microscopy in the Biomedical Sciences XXII, 1196504 (3 March 2022); https://doi.org/10.1117/12.2607590

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available