Simultaneous detection of chlorosomal bacteriochlorophylls from green sulfur bacteria and phycobilins from cyanobacteria using synchronous fluorescence scans

Anna A. Zhiltsova; Elena D. Krasnova; Dmitri A. Voronov; Galina N. Losyuk; Natalia M. Kokryatskaya; Svetlana V. Patsaeva

doi:10.1117/12.2626191

29 April 2022 Simultaneous detection of chlorosomal bacteriochlorophylls from green sulfur bacteria and phycobilins from cyanobacteria using synchronous fluorescence scans

Anna A. Zhiltsova, Elena D. Krasnova, Dmitri A. Voronov, Galina N. Losyuk, Natalia M. Kokryatskaya, Svetlana V. Patsaeva

Author Affiliations +

Proceedings Volume 12192, Optical Technologies for Biology and Medicine; 121920K (2022) https://doi.org/10.1117/12.2626191
Event: XXV Annual Conference Saratov Fall Meeting 2021; and IX Symposium on Optics and Biophotonics, 2021, Saratov, Russian Federation

Abstract

Optical characteristics of water in stratified lakes can be used to study the pigment composition of various groups of photosynthetic organisms, its depth distribution and changes in different seasons. In the Arctic zone it is especially important to explore the evolution of natural water bodies under the influence of climate change and urbanization processes. Several water bodies on the coast of the Kandalaksha Bay of the White Sea are known as being at different stage of isolation from the Sea. In some of them the colored water layers in their chemocline zone resulted from the massive development of anoxygenic phototrophic bacteria were observed. The most numerous inhabitants of the chemocline zone are green sulfur bacteria. Besides green sulfur bacteria and purple sulfur bacteria, there were found cyanobacteria above and inside the chemocline zone. To access the pigment concentration, we measured fluorescence spectra of acetone extractions prepared from water sampled from various depths within chemocline of the lake Trekhtzvetnoe in March 2021. To increase the sensitivity of fluorescence measurements to certain pigments we acquired synchronous spectra of luminescence spectrometer performing scanning with a constant wavelength offset of 30 nm. This paper presents for the first time depth distribution of photosynthetic pigments BChl d (from green sulfur bacteria) accompanied with phycobilins (from cyanobacteria) derived from synchronous fluorescence spectra of acetone extractions prepared from water from various depths. This type of spectral measurements allowed us to receive depth distribution of cyanobacteria within the bacterial plate of green sulfur bacteria with very high depth resolution.

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Anna A. Zhiltsova, Elena D. Krasnova, Dmitri A. Voronov, Galina N. Losyuk, Natalia M. Kokryatskaya, and Svetlana V. Patsaeva "Simultaneous detection of chlorosomal bacteriochlorophylls from green sulfur bacteria and phycobilins from cyanobacteria using synchronous fluorescence scans", Proc. SPIE 12192, Optical Technologies for Biology and Medicine, 121920K (29 April 2022); https://doi.org/10.1117/12.2626191

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KEYWORDS

Luminescence

Bacteria

Sulfur

Absorption

Hydrogen

Microorganisms

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