Single-shot quantitative phase microscopy in common-path configuration

Brandon Norton; Bryce Evans; Juan Viveros-Escoto; Rosario Porras-Aguilar

doi:10.1117/12.2643364

3 October 2022 Single-shot quantitative phase microscopy in common-path configuration

Brandon Norton, Bryce Evans, Juan Viveros-Escoto, Rosario Porras-Aguilar

Proceedings Volume 12223, Interferometry XXI; 122230H (2022) https://doi.org/10.1117/12.2643364
Event: SPIE Optical Engineering + Applications, 2022, San Diego, California, United States

Abstract

Quantitative phase Microscopy is an advantageous technique to retrieve 3D cell information to estimate cell dry mass and morphology. A popular method is to implement this setup in a common path configuration because it is a system robust to vibrations, and it combines phase contrast imaging with phase shifting techniques to provide high-accuracy measurements. However, this approach requires using a spatial light modulator and at least three images to calculate the phase. In our approach, we leverage the optical anisotropy of liquid crystal materials and a polarized camera to obtain four phase-shifted images simultaneously.

Conference Presentation

Citation Download Citation

Brandon Norton, Bryce Evans, Juan Viveros-Escoto, and Rosario Porras-Aguilar "Single-shot quantitative phase microscopy in common-path configuration", Proc. SPIE 12223, Interferometry XXI, 122230H (3 October 2022); https://doi.org/10.1117/12.2643364

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